Masslynx 4.12/15/2023 The results showed that two strains, CHS 2 and CHS 3 from B. The profile of metabolites present in the crude exacts was carried out by ultra performance liquid chromatography time-of-flight mass spectrometry (UPLC/Q-TOF-MS) analysis. scorzonerifolium and were authenticated by the ITS sequence and the translation elongation factor-1alpha gene (TEF-1α) sequence analysis. In this study, in order to search for SSd-producing strains, endophytes were isolated from B. Since endophytic fungi possess the natural capacity to produce the similar secondary metabolite to that of their host plants, they are promising as alternative sources of plant bioactive natural products. and exhibits many effects, such as anti-tumor, anti-inflammation and immunomodulatory. Saikosaponin d (SSd) is an important bioactive compound of traditional Chinese medicinal plant Bupleurum scorzonerifolium Willd.
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